Unicryl Formulation

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Methods for using the AGFA Duoscan Scanner

The purpose of this web site is to entice and instruct the users of the Center for Microscopy and Imaging at the University of Illinois to use the DuoScan Scanner made by the AGFA Company.

The image is captured by using an AGFA PhotoShop plug in called FotoLook. The version of Photoshop currently used in this lab is PhotoShop 4.0, put out by the Adobe Corporation

The DuoScan does far more than the average home scanner (and of course it costs more). The transparency tray actually works well with both 35mm and with the standard TEM film of 3.25 x 4 inches.

We have used this instrument for TEM film, 35mm film, Plate / Xray film, standard prints (Black and white as well as color)Also, scanning in objects placed on the top plate which results in copystand like results.

Images can even be made with protein cellulose acetate blot gels. Probably one of the most exciting finds was that we could use the scanner as a densitometer on blot gels. Especially, gels that seemed to have hardly any information in them, resulted in images that had detailed information in them. For more on this technique see the Cellulose Acetate technique and images in corresponding anchor below.

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Procedures For Different Techniques:

• Three Dimensional Objects........ Similar to Copy Stand work.
• Imaging from a Black & White Print.
• Imaging from a Color Print.
• Imaging from a Transmission Electron Microscopy (TEM) Negative.
• Imaging from a Scanning Electron Microscopy (SEM) Negative.
• Imaging from Black & White 35mm Film.
• Imaging from Color 35mm Film.
• Imaging from Cellulose Acetate Protein Blots.
• Imaging from Positive Plate Film --- RNA,DNA and Protein Blots.

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May 29th, 1997 Created by Lou Ann Miller	Center for Microscopy &Imaging
email to: lamiller@uiuc.edu	University of Illinois